thr77 login Options
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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has become observed in many tumors, which results in chromosome instability, and thus could contribute to tumorigenesis. This protein was found to affiliate with, and become linked to, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a essential function in promoting mobile-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, happen to be explained. Two added splice variants had been claimed but specific nucleotide sequence details isn't however out there.
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Blocking peptides are peptides that bind exclusively to the goal antibody and block antibody binding. These peptide commonly contains the epitope acknowledged because of the antibody.
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This guidebook outlines additional probable optimization of cellular and immunoassay parameters to make sure the best possible success are...
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This tutorial outlines further more probable optimization of mobile and immunoassay parameters to be certain the very best effects are...
Antibodies sure to the blocking peptide no more bind to your epitope to the focus on protein. By comparing the staining in the blocked antibody vs . the antibody by itself, you can see which staining is distinct.
Focus on Details Cyclin E belongs towards the extremely conserved cyclin family, whose users are characterised by a remarkable periodicity in protein abundance with the mobile cycle. Cyclins purpose as regulators of CDK kinases. Diverse cyclins exhibit distinctive expression and degradation designs which contribute to the temporal coordination of each and every mitotic function. Cyclin E forms a posh with and features as a regulatory subunit of CDK2, whose exercise is required for cell cycle G1/S changeover. This protein accumulates with the G1-S phase boundary and is particularly degraded as cells progress through S phase.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by the two antibodies...
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